Nitrotyrosine ELISA 试剂盒
Quick Overview for Nitrotyrosine ELISA 试剂盒 (ABIN5657415)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.7 ng/mL
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Nitrotyrosine (NT). No significant cross-reactivity or interference between Nitrotyrosine (NT) and analogues was observed.
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灵敏度
- 1.35 ng/mL
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Nitrotyrosine ELISA Kit
适用: 化学剂 Colorimetric Competition ELISA Plasma, Serum
Nitrotyrosine ELISA Kit适用: Various Species Colorimetric Competition ELISA 3.7 ng/mL - 300 ng/mL Plasma, Serum
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说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Nitrotyrosine (NT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Nitrotyrosine (NT) and unlabeled Nitrotyrosine (NT) (Standards or samples) with the pre-coated antibody specific to Nitrotyrosine (NT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Nitrotyrosine (NT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Nitrotyrosine (NT) in the sample.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitrotyrosine (NT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitrotyrosine (NT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 4-8 months
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- Nitrotyrosine
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物质类
- Chemical
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背景
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Gene Name: Nitrotyrosine
Gene Aliases: 3-Nitro-L-Tyrosine, 3-Nitrotyrosine
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